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1.
东海原甲藻cDNA文库构建及尝试性EST分析 总被引：2，自引：0，他引：2
东海原甲藻(Prorocentrumdonghaiense)是中国沿海频繁发生的大规模赤潮的原因种之一,大规模分离鉴定东海原甲藻功能基因是理解东海原甲藻赤潮形成过程和机理的重要基础。取对数生长期藻体,经微量总RNA抽提、cDNA合成、cDNA扩增和克隆等步骤,构建了东海原甲藻cDNA文库并进行了尝试性表达序列标签分析。从含有约5000个转化子的文库中随机取150个测序,获得126条EST序列。经网上BlastN及BlastX分析,共发现11个是已知功能的基因的标签。这些基因与东海原甲藻生长发育、物质转换和能量代谢相关。 相似文献
2.
A survey was made of the number of the heterotrophic bacteria in surface seawater of Great Wall Bay and its adjacent waters, Antarctica during the period of austral summers from Dec.1993 to Feb. 1994. The result shows that the number of the heterotrophic bacteria in the surface waterof the surveyed area is about 798 CFU. cm-3, ranging from 53 to 4 250 CFU. cm-3. Comparison of thenumbers for three months shows a declining tendency month by month. Comparing with the previous results, this one shows that the heterotrophic bacteria number changes somewhat, but slightly. It is lower than that in nearshore waters but higher than that in the open area. This shows that there is a positive correlative relationship between CFU and NH4- N, which means that heterotrophic bacteria should be an important agent in NH4-N reproduction in the environment surveyed. There is an significant negative correlation both between CFU and NOZ-N, and between CFU and PO4-P. This means that the NOZ-N, PO.-P, which were decomposed and transformed by bacteria, were used rapidly by phytoplankton. 相似文献
3.
同步N-策略多重休假的M/M/C/WV排队 总被引：1，自引：0，他引：1
考虑同步N-策略多重休假和工作休假M/M/c排队,简记为M/M/c（N-WV）。在休假期间,服务员并未完全停止工作而是以较低的速率为顾客服务。用拟生灭过程与矩阵几何解方法,给出了系统稳态队长和稳态条件等待时间的分布。此外,也得到了队长和等待时间的条件随机分解结构及附加队长和附加延迟的分布。 相似文献
4.
Performance Evaluation of a Self-Developed Ozonesonde and Its Application in an Intensive Observational Campaign 总被引：1，自引：0，他引：1 
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下载免费PDF全文 ZHANG Jin-Qiang XUAN Yue-Jian XIA Xiang-Ao LIU Ming-Yuan YAN Xiao-Lu PANG Li BAI Zhi-Xuan WAN Xiao-Wei 《大气和海洋科学快报》2014,(3):175-179
To make a detailed test on the reliability and detection performance of the electrochemical concentra- tion cell （ECC） type ozonesonde which had been devel- oped and preliminarily evaluated by the authors, an inten- sive ozonesonde release experiment was held at two sites in Beijing and Changchun in June 2013. The results showed that the mean background current and its standard deviation were 0.03 （0.04） pA and 0.02 （0.03） pA in Bei- jing （Changchun）. The average response time and its standard deviation were 27.8 s （30.4 s） and 4.0 s （3.7 s） in Beijing （Changchun）. The ozone partial pressure profiles at both sites showed a central peak in the stratosphere and a side peak in the boundary layer. Large variation in ozone partial pressure was observed at the middle levels of the atmosphere （10-17 kin）. A more marked gradient of ozone change was observed in Beijing （3.4 mPa km-1） at the lower atmosphere level, as compared to that in Changchun （0.4 mPa km-l）. The results presented in this paper indicate that this self-developed ozonesonde shows a high level of reliability and good performance. The ozonsonde is expected to play an important role in opera- tional observations of ozone profiles. 相似文献
5.
中国对虾(Fenneropenaeus chinensis)视神经节神经内分泌细胞电生理特征 总被引：2，自引：0，他引：2
利用膜片钳技术对体外培养24—48h中国对虾的视神经节端髓A型和B型神经内分泌细胞进行电生理特征研究。电流钳的结果表明，A型细胞具有自发和诱发放电活动；B型细胞没有记录到明显放电活动。在电压钳模式下，中国对虾视神经节神经内分泌细胞表达TTX敏感Na^ 通道、高电压激活L型Ca^2 通道、TEA敏感晚钾通道、早钾通道。Ca^2 通道电流在-40— -30mV被激活，在-10—0mV时达到峰值；Ca电流受钳制电压的影响。一定电压范围内，钳制电压越负，Ca电流就越大。外向的钾通道电流均在-40mV左右被激活。晚钾通道电流对TEA敏感，但TEA不能完全阻断晚钾通道电流。 相似文献
6.
Characteristics of a Negative Cloud-to-Ground Lightning Discharge Based on Locations of VHF Radiation Sources 下载免费PDF全文
下载免费PDF全文 The lightning very high frequency （VHF） radiation location system based on the short-baseline time-difference of arrival （TDOA） technique provides an effective approach to describe the temporal and spatial development of lightning discharge in two dimensions with high resolution. A negative single-stroke cloud-to- ground （CG） lightning flash was analyzed in detail using the radiation location results and synchronic fast/slow elec- tric field changes. The long-duration preliminary break- down process appeared to propagate with hi-directional leader channels. The two negative simultaneous discharge channels sloped down with a considerable horizontal com- ponent in the lower positive charge region at speeds of about 105 m s-1. The stepped leader was clearly converted from one channel of the preliminary breakdown process and spread downwards with branches. The speeds of the stepped leaders were about 105 m s -1. The K processes after the return stroke could either directly initiate from the start region with negative polarity lightning discharge, or initiate from a new region in the cloud as negative recoil streamers. All K processes propagated along the preceding electrified channel, while not all K processes initiated from the tips of positive breakdowns. The speeds of the K processes were about 106-107 m s-1. 相似文献
7.
应用PRIMER软件进行浮游植物群落结构的多元统计分析 总被引：2，自引：0，他引：2
根据青岛近海进行的一次添加营养盐的围隔实验数据,采用专业软件PRIMER中的多元统计分析方法,进行了浮游植物群落结构的变化研究。结果表明,虽然由于本实验中浮游植物的生长受到Si的限制,硅藻类群不占明显的优势,使得优势种随时间的变化不明显,没有出现明显的群落演替,但用PRIMER软件的聚类分析(Clustering)和多维尺度转换排序(MDS)分析表明,添加营养盐后浮游植物群落结构有较明显的变化,同时,PRIMER软件的相异系数(ANOSIM)分析也表明,在同时添加N、P的围隔内,浮游植物群落结构变化较只添加P的围隔中的变化显著。这显示了PRIMER软件在分析浮游植物群落结构变化和评价水域富营养化中的应用前景。 相似文献
8.
东海米氏凯伦藻水华中中华哲水蚤的选择性摄食 总被引：1，自引：0，他引：1
提要为评估中型浮游动物选择性摄食对有害藻华发展进程的影响,应用一种新的结合Frost直接摄食法和Landry稀释法的现场培养方法,于2005年4月27日—6月5日在东海有害藻华高发区的6个典型站位进行了中华哲水蚤(Calanus sinicus)对浮游植物和微型浮游动物摄食速率的研究。比较了中华哲水蚤对米氏凯伦藻(Karenia mikimotoi)和具齿原甲藻(Prorocentrum dentatum)摄食习性的差异,并评估了其摄食在水华进程中的作用。研究结果表明,中华哲水蚤对有害藻华物种存在摄食选择性和摄食速率的阈值。当自然水体中米氏凯伦藻细胞丰度达到157cells/ml和具齿原甲藻细胞丰度达到981 cells/ml时是中华哲水蚤由偏好趋于排斥摄食的阈值。当自然水体中米氏凯伦藻细胞丰度达到176 cells/ml时,中华哲水蚤对其停止摄食。米氏凯伦藻有害藻华发生区中华哲水蚤对具齿原甲藻的无选择性滤食以及对米氏凯伦藻的排斥摄食行为,影响水华进程,最终导致水华物种向米氏凯伦藻方向演替。 相似文献
9.
Depositional model of the lower-middle Ordovician Majiagou Formation in Daniudi Gas Field,Ordos Basin 下载免费PDF全文
下载免费PDF全文 Lei Tao Deng Hu-Cheng Wu Dong Fu Mei-Yan Tang Ming-Yuan Cui Lu Ding Xiao-Qi Xia Yu Xie Xin-Hui 《古地理学报》1999,22(3):523-538
Carbonate rocks are the important Paleozoic oil and gas reservoirs in the Ordos Basin. Based on the comprehensive analyses of core and thin section and logging data,the depositional subfacies,including evaporation platform,open platform and confined platform,were identified in the lower-middle Ordovician Majiagou Formation of the Daniudi Gas Field in the Ordos Basin. The characteristics of different depositional subfacies were analyzed in detail,and the depositional model of the lower-middle Majiagou Formation in the Daniudi Gas Field controlled by sea level fluctuation was established. The sea level was relatively low during the depositional period of the Members 1-3 of the Majiagou Formation,resulting in the development of evaporation platform. The saline lake of the Members 1 and 3 of the Majiagou Formation was mainly distributed in the southeast with the gypseous lake around it. The sea level rose slightly during the depositional period of the Member 2 of the Majiagou Formation,and subsequently muddy-dolomitic flat and gypseous-dolomitic flat developed in the entire study area from bottom to top. The open platform was developed in the Member 4 of the Majiagou Formation when the sea level reached the maximum,and uniformly distributed lime flat and overlying dolomitic-lime flat covered the whole area in the platform. The confined platform predominated the Member 5 of the Majiagou Formation during the regression-transgression-regression. In the Member 5 of the Majiagou Formation,the dolomitic flat of the 7th-10th sub-members covered the whole area,the large extent of muddy-dolomitic flat expanded from northwest to southeast in the 6th sub-member,and the lime flat of the 5th sub-member was developed in the central part with the scattered dolomitic flat around it. 相似文献
10.
碳酸盐岩是鄂尔多斯盆地古生代重要的油气储集层。综合利用岩心、薄片和测井资料,在鄂尔多斯盆地大牛地气田奥陶系马家沟组中下组合识别出蒸发台地、开阔台地和局限台地沉积亚相类型,详细分析了不同沉积亚相特征,建立受海平面升降控制的大牛地气田马家沟组中下组合沉积模式。马一段至马三段沉积时期海平面较低,沉积了蒸发台地相,其中马一段和马三段的盐湖微相主要分布于东南部,膏湖微相分布于盐湖外围,而马二段海平面略有上升,随之沉积了覆盖全区的泥云坪微相和膏云坪微相;马四段形成于最大海泛期,沉积开阔台地相,自下而上依次形成覆盖全区、稳定分布的灰坪微相和云灰坪微相;马五段形成于海退—海侵—海退过程,主体沉积局限台地相,马五7-10亚段的云坪微相覆盖全区,马五6亚段自西北往东南发育大面积的泥云坪微相,马五5亚段中部以灰坪微相为主,云坪微相围绕灰坪分散分布。 相似文献